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The cells were gently resuspended with a micropipette every 10 min
Species Human Accession P41240 Amino Acid Sequence M1-L450(end)
and 1-2mL of 4 ° C organoid subculture buffer G was added to each well for 2min
Host Rabbit Antigen MEK1 Synonyms Dual specificity mitogen-activated protein kinase kinase 1
PDCD1LG2 Accession Q9WUL5 Amino Acid Sequence Leu20-Arg219
Mouse TNF-alpha Protein, His tag DNA Immunoprecipitation The cells were gently resuspendedProduct Specification Species Mouse Synonyms Tumor necrosis factor, Cachectin, Cachectin, Tumor necrosis factor ligand superfamily member 2 (TNF a), Tnf, Tnfa, Tnfsf2 Accession P06804 Amino Acid Sequence Protein sequence (P06804, Leu80 Leu235, with C His tag) LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL Expression System HEK293 Molecular
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