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increase the centrifugation speed from 300×g to 350×g and extend the centrifugation time to 8 minutes to enhance neuron precipitation
Vortex vigorously for at least 15 minutes before use — incomplete reconstitution is one of the most common sources of false results in BET workflows
Do not pour all at once quickly—this causes the local DMSO concentration to drop from 10%-20% to 2%-4% instantly
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Component Pack Size SDS1 (Skin Dissociation Solution 1) 10 × 1
Bst DNA Polymerase, Full Length RNA Extraction increase the centrifugation speed fromBst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus that contains the 5' → 3' polymerase activity and the 5' → 3' exonuclease activity. This polymerase also has strand displacement capabilities making it an ideal candidate for isothermal amplification. Applications Isothermal amplification (LAMP, RCA) DNA strand displacement amplification Primer extension Unit Definition One unit is